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NGS Course: RNA-seq data analysis, 2015 (5th edition)

September 7, 2015 - September 9, 2015

Date: September 7-9, 2015 (5th edition)

Location: Leiden University Medical Center, The Netherlands

Course coordinators: Peter-Bram ‘t Hoen (LUMC), Jan Oosting (LUMC) and Celia van Gelder (Radboudumc/NBIC)

Course description:
The BioSB research school and partners LUMC and ErasmusMC are organizing a 3-day course on RNA-seq data analysis from September 7-9, 2015. This is an advanced course for people with experience in NGS. The course will consist of seminars and hands-on command line, Galaxy and R practicals and will cover the analysis pipelines for differential transcript expression and variant calling. Examples will be taken from human and mouse studies. The course does not cover prokaryotic RNA profiling nor plant- and metagenomics aspects.

Course topics:
– RNA-seq experimental approaches
– Alignment and de novo assembly
– Statistics for differential gene expression
– eQTL analysis and allele specific expression
– Variant calling and RNA editing
– Fusion transcript detection
– Small RNA profiling
– Software for RNA-seq data analysis

Preliminary programme and course flyer:
A preliminary course programme can be found here.
A course flyer can be found here.

Target audience:
Participants for the RNA-seq course should preferably have participated in the general NGS course or otherwise have demonstrated hands-on experience with NGS data analysis. The course is aimed at PhD students and post-docs, but scientific programmers and data analysts with a background in biology and bioinformatics may also attend. Registrations will be handled on a first-come-first-served basis but experience and motivation will be reviewed. Seats have been reserved for participants in the FP7-funded project RD-Connect and Marie Curie European Training Network TranCYST.

Travel and lodging:
Hotel arrangements will be made with hotels at walking distance (3 minutes walk) of the Leiden University Medical Center. Additional information will follow shortly.

Registration & information:
You can register for the next course (planned for 7-9 September 2015) via the enrollment page.


September 7, 2015
September 9, 2015


Elizabeth Vroom


Daniel Renault
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